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specifically the mobile and stationary phases

2007-10-06 08:45:06 · 2 answers · asked by ? 4 in Science & Mathematics Chemistry

2 answers

Thin layer chromatograpy is stationary at all times during the process. Gas chromatography uses a inert gas (helium or nitrogen) to pass the sample through the machine.

According to Wikipedia: http://en.wikipedia.org/wiki/Thin-Layer_Chromatography
"Thin Layer Chromatography (TLC) is a chromatography technique used to separate chemical compounds [1]. It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose immobilised onto a flat, inert carrier sheet. A liquid phase consisting of the solution to be separated is then dissolved in an appropriate solvent and is drawn up the plate via capillary action, separating the experimental solution based on the polarity of the components of the compound in question.

Its wide range of uses include:
- determination of the pigments a plant contains
- detection of pesticides or insecticides in food
- analysis the dye composition of fibers in forensics, or
- identification compounds present in a given substance

It is a quick, generic method for organic reaction monitoring."

According to Wikipedia: http://en.wikipedia.org/wiki/Gas_chromatography
"Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen, and the stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside glass or metal tubing, called a column. The instrument used to perform gas chromatographic separations is called a gas chromatograph (also: aerograph, gas separator)."

2007-10-06 08:52:15 · answer #1 · answered by Dan S 7 · 0 0

There are various strategies for chromatography which may be used in line with what you try to cut up/isolate/become attentive to. between the simplest could be paper chromatography, the two ascending or around, in line with solvent separation regarding a mix of Butyl Alcohol : Acetic Acid : Water interior the ratio 4:a million:5, you will discover protocols dotted around the interweb. i'm uncertain what you advise by using table right here, could desire to you be clearer?

2016-11-07 11:03:17 · answer #2 · answered by ? 4 · 0 0

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