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2007-10-03 03:52:21 · 2 answers · asked by Anonymous in Science & Mathematics Chemistry

2 answers

I am not sure about your question but probably you are asking for 'brightfield microscopy" as opposed to "darkfield microsopy".

Brightfield microscopy is the simplest and most common of the microscopic techniques. It is when the specimen is illuminated from below and seen from above through the ocular lenses. This is basically what you do in typical microscopic work. On the other hand, Darkfield microscopy is used for specimens that are very transparent. The illumination is specially reduced to create a "darkened" contrast. One example of this technique is the so called Rheinberg illumination. It is a variant of dark field illumination in which transparent, colored filters are placed just before the condenser so that light rays at high aperture are differently colored than those at low aperture.

Then, I believe 'Bright microscope' means that the microscope is used for brightfield microscopy work. So I am not sure if there is another term for it. But you may just call it "optical reflecting microscope"

2007-10-03 04:04:52 · answer #1 · answered by Aldo 5 · 1 0

Brightfield microscopy is simple when the illuminator's light, from halogen or tungsten bulb, is unaltered both before and after hitting the specimen. The light path cannot be partially blocked or distorted on its way to or from the sample by phase contrast annuli, darkfield stops, or DIC prisms, or by polarizing or fluorescence filters, or by ultraviolet light bulbs. Brightfield microscopes can be upright or inverted scopes with light path from either above or below, and often use with specimen are stained, contrast with the background or mounting medium in color to increase the effectiveness of view.

It is also called metallurgical microscopes to examine opaque specimens, which will not transmit light, when using with half mirror as a beam splitter.

2007-10-05 06:19:14 · answer #2 · answered by toodd 4 · 0 0

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