I need something to help me grow yeast
2007-10-02 10:05:07 · 4 answers · asked by Uryuu Ishida 1 in Science & Mathematics ➔ Alternative ➔ Other - Alternative
* To make 1 liter of YD media (also called YEPD and YPD):
1% Yeast Extract (Bacto) = 10 g per liter
2% Bactopeptone = 20 g per liter
2% Dextrose (glucose) = 20 g per liter
Place ~500ml of H2O and a stir bar into the flask
Stir on stir plate until all goes into solution (note if making plates agar will NOT go into solution)
Transfer to graduated cylinder. Add H2O to 1L. Pour back into 2L flask.
Cover flask with aluminum foil. Add small piece of autoclave tape. Autoclave at 121° C for 25 min, liquid cycle (slow exhaust).
Media will caramelize (turn brown due to "cooked" sugar) if left in autoclave too long. Slight caramelization will not hurt the media.
Allow media to cool then store in refrigerator or use when room temp.
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* To make 1 liter of YES media:
Yeast Nitrogen Base with Amino Acids = 6.7 g/l
Dextrose (glucose) = 2.0 g/l
Sodium Succinate = 10.8 g/l
Yeast Extract = 1.0 g/l
(Note: To make liquid growth media into solid media for petri dish plating add 2% agar (20 g/l)).
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2007-10-02 10:55:41 · answer #1 · answered by Hanna 2 · 2⤊ 1⤋
Yeast is microscopic which means it can only be seen while looking in a microscope, because it is so small. The scientific name for baking yeast is Saccharomyces cereviae. Yeast is alive and needs warmth, sugar and water to stay alive. It can use several things with sugar in it to live like regular table sugar, honey, molasses, maple syrup and fruit. As the yeast grows, it produces carbon dioxide which we can see as tiny bubbles in the mixture. After the yeast starts to grow, a baker adds flour and salt and other ingredients to bake bread. Those same carbon dioxide bubbles continue to grow and cause the bread dough to grow or 'rise.' The bubbles of gas make the dough get bigger and bigger.
2007-10-03 00:43:46 · answer #2 · answered by kokopelli 6 · 1⤊ 0⤋
Have you tried the chemstry area? This section is populated mainly with the ghosties.
2007-10-02 17:29:42 · answer #3 · answered by Peter D 7 · 0⤊ 1⤋
corn syrup and warm distiled. keep it around body temperature. Make sure you have clean glassware with on soap residue.
2007-10-02 21:08:59 · answer #4 · answered by John S 5 · 2⤊ 0⤋