when you try to observe the organism by microscope, do you see the opposite direction of their action?

Question

when you see something moving in the microscope like to the top and the the right, do you really see the opposite of that through the eyepiece? and second, why do we have to focus the low-power objective first?

Answer 1

It depends on the design of the microscope. They can be designed either way. Focusing first at low power is easier and faster, you can focus on the correct part of the sample, and you are less likely to drive the high power objective through the sample, breaking the sample, the slide, and the expensive objective lens.

Answer 2

To see the opposite direction of the object from the bottom, use an invert microscope. It is easy to get a big picture of the specimen, focus, center or select a specific object of the specimen with a low magnification objective first, then simple turn the objectives to higher level of optical magnification to observe without need to adjust the stage.