Why can samples of solutions often be separated into their compenents by chromatography?
I'm not sure how to explain this. Can anyone help PLEASE thanks sooo much!!!
2007-09-27 02:38:43 · 4 answers · asked by Natlsn12009 1 in Science & Mathematics ➔ Chemistry
it is simply based on interaction of a molecule with other (you might call it affinity to other molecule).
Basically you'll have a stationary phase and mobile phase. The mobile phase of course contains the molecule you want to separate. This mobile phase flows through the stationary phase using carrier medium which do nothing other than to transfer your samples through the stationary phase. every molecule in the sample have a different affinity to the stationary phase, the higher the affinity the more it will be attract and stay with the stationary phase thus the longer time it will take before it goes out completely from the stationary phase (high residence time) the opposite is true for substances that have lower affinity with the stationary phase e.g. low residence time. This is how at the end you could separate a mixture of samples into their building components
Depending on what you want to separate, you could use different molecule-molecule interaction properties and use it as your stationary phase. The most common is to differ the molecule-stationary phase affinity by polarity. You could as well use chirality as your interaction criteria and many other.
Depending on the mobile phase you could have it as a gas phase or liquid phase chromatography.
hope it would help you a bit to understand the underlying principle in chromatography.
2007-09-27 02:52:36 · answer #1 · answered by IonicLiquids 2 · 0⤊ 0⤋
Beacuse the components are different chemically,so they move at different rates over the stationary phase. The components are like horses, and the stationary phase is like a race track. All the horses start at the same gate at the same time, but as time goes on, they get separated from one another in come in in acertain order.
2007-09-27 02:49:42 · answer #2 · answered by steve_geo1 7 · 0⤊ 0⤋
skinny Layer Chromatography (TLC) is equivalent to Paper different than rather than employing a stationery component of paper it makes use of a skinny layer of adsorbent (silica gel or cellulose) on a flat, unreactive substrate. that's subsequently extra perfect because it runs swifter and has clearer separations
2017-01-02 18:16:29 · answer #3 · answered by ? 3 · 0⤊ 0⤋
i dont know
2007-09-27 02:47:03 · answer #4 · answered by arunkumar 1 · 0⤊ 0⤋