is the gram stain of significant importance in the identification of the organism studied?

Answer 1

Yep! You need to identify if the unknown is either gram positive or negative, because then you can eliminate two other stains depending upon if it is gram negative.

Answer 2

theory of Gram's Stain The crystal violet stain is the known stain, which stains each and every thing interior the smear blue. The Gram's iodine acts as a mordant that reasons the crystal violet to penetrate and cling to the gram-effective organisms. The acetone-alcohol mixture acts because of the fact the decolorizer that washes the stain far from each and every thing interior the smear different than the gram-effective organisms. The safranine is the counter-stain that stains each and every thing interior the smear that has been decolorized: pus cells, mucus, gram-detrimental organisms. The gram-detrimental organisms will stain a lots deeper purple than the pus cells, and mucus will stain even lighter purple than the pus cells.

Answer 3

Yes.
1. It is useful in differentiating gram positive and gram negative bacteria
2. morphology of bacteria , whether it is cocci or bacilli
Bacteria like pneumococci, meningococci and gonococci can be demonstrated
3. Based on the back ground, number of pus cells inflammatory condition can be identified.
acute and chronic condition can be differentiated based on type of cell
4. Emergencies like meningitis,preliminary diagnosis can be given and emperical treament can be started
5. Anaerobic organisms can be seen in gram stain. Unless specimen collection and appropriate media and culture method is used for isolation of anaerobes. Any failure in isolation diagnosis is entirely based on gram stain
6. Fastidious organisms which can not be isolated easily
7 to select the appropriate culture medium
8. to select antibiotics for antibiotic sensitivity testing
9. severity of infection
10. to differentiate pathogens from commensals
11. first step in identification of organism

Answer 4

It is very important actually. The reason for this is not to compare how other bacteria stain, but that the stain will tell you what kind of cell wall the bacteria have. Once you know that piece of information you can eliminate a wide group of possible species.

Answer 5

Yes, knowing if an organism is gram-positive or gram-negative is one of the first steps in identifying bacteria.

Answer 6

Yes it is. If you find out if the organism is gram-positive or gram-negative, your work will be a lot easier as you can rule out many posibilities.

Answer 7

It's used for bacteria...

http://en.wikipedia.org/wiki/Gram_staining