what methods of assay are are there for a protein?

Question

more specifically proteins that are not an enzyme.
thanks

Answer 1

Quantitative assasy

if you have a pure sample :
Go for Lowry's method of assay

if the sample is contaminated by some ions or other chemicals :
then go for Bradford's method of assay beasuce it is not affected by contaminants , but the limit of detection is low when compared with lowrys ( limit of detection is 20 micro grams).

Qualitative assay:
Native PAGE ( does not denature protein)
SDS PAGE ( denatures it )
if you just need to check the presence of protein , then carry out acid hydrolysis and carry out ninhydrin test ( best for amino acids )

Answer 2

Quantitative assays? The Bradford and the Lowry assay are the most commonly used methods. There are various reagent kits you can buy for them. Like pretty much all colorimetric assays, they depend on comparing the sample color intensity to a standard curve of intensity vs known concentration. There are some other methods as well, but the problem with all these is that they tend to not work very well in the presence of thio- compounds, so for 2D electrophoresis and such, you often end up doing a gel just to get a visual comparison to a known qantity or to standardize all your samples at the same amount.

Answer 3

SDS-PAGE gel, polyacrylamide gel electrophoresis.
Or, Immunoassays... for non enzyme immunoproteins.

Answer 4

electrophoresis