Can you fuse dna?

Question

How can you fuse DNA?

Answer 1

Use sedai virus to fuse two cells.
Use DNA ligase to connect two pieces of DNA.

Answer 2

it's called DNA recombination.

restriction enzymes separate the hydrogen bonds between nucleotides to create what are called "sticky ends"
you can use restriction enzymes to cut a piece off of your first DNA strand and also to open up your second DNA strand at the point of insertion.
then, the enzyme DNA Ligase reconnects the nucleotide bonds (those between your old piece and your new piece).
think of the game Tetris... the block that is slightly Z shaped. that is your DNA fragment with sticky ends. the DNA that will be accepting this fragment will be cut so that it will match up with your nearly Z-shaped fragment. the DNA ligase bonds them together and you have a piece of recombinant DNA!

now, once you have this new DNA, it is pointless unless you create lots of it. usually if you are interested in recombining DNA you introduce the fragment into a bacteria. Bacteria have plasmid (or circular shaped) DNA. it's not very long or coiled, since it's just a simple bacteria. so once you insert this fragment into the bacteria's DNA, you can put the bacteria into a growth medium, that is, an environment that supports a bacteria's exponential growth. so then your new DNA will just keep multiplying until you have enough. then you purify and extract what it is that you need.
>>don't ask me how to do that, cause i'm no scientist here. i think it's different depending on what the DNA you inserted is coding for.

Answer 3

I don't know exactl what you are talking about, but here goes.

If a single stranded DNA molecule is placed into a solution with its reverse compliment (remember 5' - 3' bind to 3' - 5', A to T, G to C?) it will "fuse" on its own to create a double helix.

If two pieces of DNA have matching overhands (as if they had been cut with similar restriction enzymes), DNA ligase can be added, and that will take the two cut pieces and put them back together, almost like repairing a broken chain.

I hope at least one of these answered your question.

Answer 4

Yes you can
you use restriction enzymes to cut the DNA at specific points along the helix. you usually end up with overhanging 5 prime or 3 prime ends (called sticky ends) - you can then insert a foreign piece of DNA so that it fuses (anneals is the correct term) with these sticky ends.............that is how you insert a piece of DNA into another piece of DNA!!!!


hope that was a simple explanation

Answer 5

The original way of fusing DNA is of course through the process of reproduction. If you can simulate this process in a lab, you can say you fused DNA

Answer 6

yes, you use this enzymes called restriction endonucleases or molecular scissors, they cut the DNA, so you can cut two DNAs from diferent origen with the same enzyme, then put them together and they're going to join because of the complemetary of the nitrogen bases, then you use another enzyme, the DNA ligase to finish the fusion(creating the links between the ribose and the fosfate)

Answer 7

use you sure can. with the help of protein enzymes. i cant name any off the top of my head, but I know there are proteins that make cuts along the DNA, and allow for insertions of strands of DNA.

Answer 8

What do you think "Frank'nFood" is.

The question is should you fuse DNA. Answer... Not in a lab. Do it the old fashion way, birds, bees, its more fun!!

Answer 9

Intercourse?