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my assignment is to draw a restriction map of the fragment and show distances, in base pairs, between HindIII, SmaI, and EcoRI.
A 1000bp EcoRI restriction fragment (linear) contains a gene you are interested in. first, construct a restriction map of the fragment using the enzymes SmaI & HindIII.
HindIII cut at 700bp & 300bp; SmaI cut at 800 & 200.

2007-09-07 16:22:45 · 1 answers · asked by LINDA 1 in Science & Mathematics Biology

1 answers

It is particularly helpful in chopping copies of tandemly arranged copies of a gene. For example, you can get restriction fragments of ribosomal RNA genes resident in the nucleolus. You can measure the lengths of various restriction fragments in the EM.

2007-09-11 16:07:42 · answer #1 · answered by Ishan26 7 · 0 1

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