I have a dilution question.?

Question

I need to make 1:50 dilution but I need 6ml how do I do that?
I also have a stock solution of 1:1000 so 2ul into 2ml how do I make 1:10000,1:20000,1:30000,1:40000, 1:50000 and 1:60000 with this solution.

Answer 1

Divide the 6 ml by 50 for 0.12 ml.
Add 120 ul to 5.88 ml
The ratio symbol is just another way to note a fraction.
This solution has 50 parts but only one part is the solute.

For the next you are making serial dilutions.
2ml divided by 10^3 = 2ul added to 1,998ul
or
Do 2ul into 198 for 1:100 (Check by 198/2=99)
Then go 200 ul into 1800ul for 2ml at 1:10^3 (1800/200=9)

Now take 100 ul into 900ul for a ten fold dilution step.1:10^4

From the 1:10^4 do a series
1:1, 1:2, 1:3, & 1:4 etc

Take (original conc.) / (dilution factor) = new conc.

Answer 2

this is the general rule of thumb..

you take your desired final volume.. in this case.. 6ml...
if you want a 1:50 dilution, then you take 6/50 = 0.12
that means you need to add 0.12ml of the solution you want to dilute to 6-0.12 = 5.88ml of diluent.

if you want to go from a 1:1000 stock to 1:10000 then just dilute 10 times..

1:20000 then dilute 1:1000 stock 20 times.

1:30000, dilute stock 30 times.

etc