Does anyone know this?

Question

what is meant by the optimum pH of enzyme activity in terms of enzyme charge?

Answer 1

not me sorry

Answer 2

most enzymes work best at a pH that is physiology for them, so e.g. most proteinases that are expressed anywhere inour body like more or less neutral pH best (around 7.0 some like it slightly basic 7.4 or so). However trypsin, which is expressed in our stomach, where th physiologic pH is arouond 2.0 is adapted (through evolution) to work best at this highly acidic pH. The way to find out is to measure enzyme activity at differnt pH levels, you'll notice that each enzyme has a optimal pH at which it is most active. This actual charge at the pH optimum differes from enzyme to enzyme, but it is true that all enzymes since they are proteinss have some basic and some acidic amino acid residues, they carry charges or are neutral depending on the pH (in general acidic amino acid residues are charged at higher pH, while basic ones are charged at lower pH)

Answer 3

The answer is explained in this website i guess you will find it hard to understand so read it peice by piece break it down and you will get there.

"Enzymes are amphoteric molecules containing a large number of acid and basic groupsmailny found on their surfaces.
The charges on these groups will vary, according to their acid dissociation constants (Dissociation in chemistry is a general process in which ionic compounds separate or split into smaller molecules, ions, or radicals, usually in a reversible manner).This will effect the total net charge of the enzymes and the distribution of charge on their exterior surfaces, in addition to the reactivity of the catalytically active groups.These effects are especially important in the active sites.Taken together, the changes in charges with pH affect the activity, structural stability and solubility of the enzyme."
-all from the below link and definition from wikeopidia

Good luck its been a couple of years since i did chemisty and biology so i had to get some help from a site xD

Answer 4

depending on the pH, the conductivity of the enzyme will differ.

Answer 5

its tough without knowing the context of your question, but i think they mean that if a molecule is positively charged, in general it will give off protons (H+) and therefore be more acidic (lower pH) while negatively charged molecules in general have an excess of electrons and will be more basic (higher pH)

Answer 6

Woohhaaa far too deep for me.....!