In lab we did a DNA extraction from a strawberry. We got a lecture on it, but the one thing I can't remember is how the detergent lyses the cell. Can some one explain that to me?
thanks
2007-07-04 15:09:40 · 4 answers · asked by blackcat3556 4 in Science & Mathematics ➔ Biology
Detergents lyse the cells by rendering the outer bilipid layer soluble. This is achieved by a process called micelle formation.
When excess of the detergent is added it incorporates the lipids in its hydrophobic core and forms a sort of circular structure with the hydrophilic groups facing the solvent ( water) and the hydrophopic core facing inwards. This is how it solubilizes the lipid bilayer.
2007-07-05 04:07:20 · answer #1 · answered by banshee2327 2 · 0⤊ 0⤋
The detergent disrupts the cell membrane by solubilizing the lipid bilayer. I imagine you had to grind the tissue as well though, that would take care of the cell wall.
2007-07-04 15:19:40 · answer #2 · answered by Anonymous · 2⤊ 0⤋
or the DNA donor could be genetically predisposed to alcoholism... sorry I'll shut up now.
2016-04-01 08:06:21 · answer #3 · answered by Anonymous · 0⤊ 0⤋
Gentle shaking and use of blender will do.You can shake up side down or use shaker.
2007-07-04 16:38:23 · answer #4 · answered by Ishan26 7 · 0⤊ 0⤋