Will any type of mRNA show up as bands in a Northern Blot?
2007-06-20 04:35:53 · 4 answers · asked by Anonymous in Science & Mathematics ➔ Biology
Any type would show up. However, since you'd probably want to compare different mRNAs on as even a level as possible, you'd want to have mature RNAs. Introns can significantly change the size of the mRNA molecules, so you wouldn't get accurate or precise results with pre-mRNA.
2007-06-20 05:39:52 · answer #1 · answered by Sci Fi Insomniac 6 · 0⤊ 0⤋
The only thing you need to detect an RNA in a Northern is complementarity to your probe. The gel dosn't know if it's pre-mRNA or not.
Are you looking for some sort of alternative splicing project? I don't know why you're going after pre-mRNA. Ask again - there may be a better route to the information you want.
2007-06-21 08:01:16 · answer #2 · answered by michaelhobbsphd 3 · 0⤊ 0⤋
As far as I know, you can electrophorese either mature mRNA or pre-mRNA and probe it in a northern blot.
2007-06-20 11:45:56 · answer #3 · answered by hcbiochem 7 · 1⤊ 0⤋
I agree, I'm pretty sure, when I was learning about it in class, my teacher did not specify, he just said mRNA...
2007-06-20 12:38:17 · answer #4 · answered by Tropical Mango 2 · 0⤊ 0⤋