what should be blank reading in estrase enzyme assay?

Question

enzyme essay by titration with 0.1 N NaOH using substrate contain ethyl butyrate 1ml and 0.3M potassium Phosphate buffer pH 7.0.and 5 drops of 1% phynolphthelein .

Answer 1

The blank reading needs to be obtained by carrying out the titration without adding the substrate and performing the titration by adding all other required reagents. The neutralisation value obtained will be the blank reading.

Answer 2

Data required.