Ethidium bromide is the dye.
It binds to the DNA, and then when exposed to ultraviolet light, it fluoresces.
Ethidium bromide tends to split the chains of the DNA, and one can absorb it through the skin, which is why it is essential to wear gloves when dealing with agarose gels that have been treated with it.
2007-06-16 05:31:42
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answer #1
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answered by kt 7
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Ethidium bromide is commonly used to detect nucleic acids in the lab. In the case of DNA this is usually double-stranded DNA from PCRs, restriction digests, etc. Single-stranded RNA can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate. Detection typically involves a gel containing nucleic acids placed on or under a UV lamp. Since ultraviolet light is harmful to eyes and skin, a camera is used for recording ethidium bromide fluorescence. In other cases, a protective screen is put between the observer and the UV source. In the cytogenetic laboratory the intercalating properties have long been utilized to minimize chromosomal condensation when a culture exposed to mitotic arresting agents during harvest. The resulting slide preparations permit a higher degree of resolution and thus confidence, in determining structural integrity of chromosomes upon microscopic analysis.
2007-06-16 23:35:17
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answer #2
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answered by Anonymous
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ethydium bromide is good for light microscopy, useless for electron microscopy.
Uranyl acetate and lead citrate are the traditional dyes for electron microscopy. Both bind to DNA and RNA.
2007-06-16 14:53:20
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answer #3
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answered by Anonymous
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for electron microscopy the dyes used are heavy metal salts such as lead citrate,lead tartarate,etc.
2007-06-17 00:47:21
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answer #4
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answered by Anonymous
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