2007-05-17 04:43:08 · 5 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
HPLC. Repeated equilibrium phase partitioning.
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"theoretical plate" HPLC theory 862 hits
2007-05-17 04:48:41 · answer #1 · answered by Uncle Al 5 · 0⤊ 0⤋
HPLC-High Performance Liquid Chromatography-is used to separate out miligrams of organic compounds dissolved in a particular solution by passing this solution though a column (~10 mm diameter) at pressures greater than 1000psi. The constituents have differering affinity for the column are detected at the outlet using fluorescence, UV, or refraction detectors. The technique is used to get purity information, and sometimes for extracting a couple of miligrams of a pure unknown compound for chemical identification by NMR or other technique.
2007-05-17 09:21:25 · answer #2 · answered by Shawn A 3 · 0⤊ 0⤋
HPLC - High Performance Liquid Chromatography is a method used to identify chemical liquid components. The principle is similar to NMR and IR (assuming you've taken Organic Chem). There's another process called GC (Gas Chromatography) where the analysis is carried out on a specimen put into gaseous form.
Hope this helps.
2007-05-17 04:50:14 · answer #3 · answered by crusfornixus 3 · 0⤊ 1⤋
HPCL - Waters Corporation develops high performance, value-added, system-based products that improve laboratory effectiveness for pharmaceutical, life science, environmental, food and beverage, agriculture and industrial applications.
2007-05-17 04:53:19 · answer #4 · answered by tictac 4 · 0⤊ 1⤋
You mean HPLC, it is based on the principle of selective adsorption.
2007-05-17 05:27:59 · answer #5 · answered by ag_iitkgp 7 · 0⤊ 0⤋