What is inserting DNA into a bacterial cell to produce insulin called?

Answer 1

Molecular cloning - The essence of molecular cloning is the propagation of a piece of eukaryotic (or prokaryotic) DNA in another cell. Usually, bacterial cells are used for this purpose because of their rapid rate of growth. This is part of Recombant DNA technology. Recombinant DNA Technology refers to molecular techniques that are used to insert DNA (genes) from one type of organism to another. For instance, the human gene for insulin production can be inserted into the DNA of a bacterial cell. The bacterial cell will then divide to produce many new bacterial cells, each with the gene for human insulin faithfully replicated. The bacteria then produces human insulin that can be harvested and used to treat people with diabetes.

http://amiga1.med.miami.edu/Medical/Werner/lecture11.htm
http://www.life.uiuc.edu/bio100/lectures/f06lects/18f06clonerecopcr4recodna.html

Answer 2

transfection or transformation
ransfection describes the introduction of foreign material into eukaryotic cells. Transfection typically involves opening transient pores or 'holes' in the cell plasma membrane, to allow the uptake of material. Genetic material (such as supercoiled plasmid DNA or siRNA constructs), or even proteins such as antibodies, may be transfected. Transfection is frequently carried out by mixing a cationic lipid with the material to produce liposomes, which fuse with the cell plasma membrane and deposit their cargo inside. The term transfection is most often used in reference to mammalian cells, while the term transformation is more often used for the same process in bacteria and, occasionally, plants.

Answer 3

relies upon on the size you prefer to offer. For great small you opt for: a million. The gene of interest spliced into your host organism. 2. A shaking incubator to start a commencing subculture for say 250mL. 4. A mechanism to burst or lyse the cells. 5. Small centrifuge to seperate cellular debris. Bingo, you get carry of a few tough protein soup. Purify if mandatory. Assay/study to make certain out what you get carry of! Its fairly low priced too.

Answer 4

You need a simple answer. It is an example of genetic engineering!

Answer 5

This is an example of genetic engineering. I'm not sure if you want something more specific then that.