How r we able 2 see the interior of a cell using light microscopy?

Question

If the bacterial cell is a 3 dimensional structure,being surrounded by the cell wall on all sides(unlike the 2D figure we see in our books),then how is dat we r able to see the stained or unstained components of the cytoplasm from the outside of the cell wall using a light microscope?

Answer 1

You cut the incoming light by narrowing the aperture and by adjusting the condenser of the light microscope. In phase contrast microscope, the incoming light is cut further by phase plate and this adds to the contrast. When you fix the cell with fixative, the refractive index changes and the organelles become better visible with the help of suitable dyes. In sections for electron microscopy you slice the cell very thin( 50 Angstrom) so that the fluorescent screen gets a better shadow the cell and organelles.

Answer 2

I doubt very much that you can actually see "unstained" components of the interior of the cells...if anything, perhaps the golgi apparatus of the eukarioyic cell, ,,,however, that is why, Giemsa, Wright, Ziehl nielsen, and other guys, took the good pathway and decided that, a tinction that stains different structures with different colors, according to its chemical composition, would be able to absorb differently, one substance or colorant, or a mixture of those (like hematoxiline-eosine, according to its acidity or alkalinity). and of course, the light will transverse the cell wall of the bacteriae, or the membrane of eukariotic cells, with a wide and different array of colors in the organelles, because they have different chemical composition.,,,,,By the way, the sample you see in the microscope (ligh, either normal or polarized),is still a 2D image....or isnt it?
Besides, the thickness calculated to be cut with the microtome, leaves slices of tissue so thin, (8-|9 millimicres) that you can see the light getting through those structurres without difficulty................

Answer 3

1.satin the specimen
2.slice it
3.feeze the specimen
4.light is able to pass through the cell structures are thus visible

Answer 4

The sections are thin and selective stains are applied. I mean really thin.

Answer 5

the sections are thin enough to where you can see through them if light is applied. and with selected dies it becomes easier to see the fetures.