what was the protein concentration of the undiluted unknown?

Question

Aliquots of a 0.5 mg/mL standard of BSA were used to construct a standard curve for the Bradford protein assay. The tubes contained the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 µL. the corresponding absorbance after adding Bradford reagent were the following: 0, 0.05, 0.09, 0.14, 0.19 and 0.22. If you took 20 µL of an unknown and added 80 µL of water, mixed, took 10 µL of the mixture, added this to the Bradford reagent, and saw an absorbance of 0.08, what was the protein concentration of the undiluted unknown?

Answer 1

33