The extinction coefficient for NADH is 6220 M-1 cm-1 at 340 nm. Calculate the following:
a. The absorbance of a 2.2* 10-5M sample in a 1-cm cuvet at 340nm.
b. The absorbance of a 2.2* 10-5M sample in a 1-mm cuvet at 340nm.
c. The absorbance of a 1mM sample in a 1-cm cuvet at 340nm.
2007-04-26 23:36:05 · 3 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
If I'm not mistaken, Absorbance (A) is equal to the product of the extinction coefficient (e), the length (b) and concentration (c). That is A=ebc.
To find the absorbance in each, just multiply all the given data with the extinction coefficient of NADH. Just remember to convert mm into cm (for question 2) and mM to M (for question 3).
2007-04-26 23:47:39 · answer #1 · answered by arjay 2 · 0⤊ 0⤋
Use the Henderson eqn.
2007-04-26 23:43:23 · answer #2 · answered by ag_iitkgp 7 · 0⤊ 0⤋
i m weak
2007-04-26 23:38:50 · answer #3 · answered by Anonymous · 0⤊ 0⤋