Here's the website.
http://www.theoscarspot.com/index.php?option=com_smf&Itemid=49&topic=2650.0
and here's the content of the website to which I'm referring.
A. Primary euthanasia methods for fish (methods that can be used alone) include:
1. MS-222/TMS- dosage: 50 to 250 mg/L
[buffered to pH 7.0 with sodium bicarbonate (NaHCO3)]
Note: Fish euthanized with MS-222 cannot be used for human consumption.
2. Benzocaine-dosage: 50 to 500 mg/L
Note: Fish euthanized with Benzocaine cannot be used for human consumption.
3. Exposure to a solution saturated with carbon dioxide (CO2):
When possible, carbon dioxide should be used from a compressed gas cylinder source. However, neither the 1993 nor the 2000 Report of the AVMA Panel on Euthanasia address the issue of the source/production of carbon dioxide as it relates to aquatic animal species. The use of chemical methods for the production of carbon dioxide, such as a saturated solution of sodium bicarbonate or Alka-Seltzer ®, has been proven quite effective with fish as noted in the literature. When dosed adequately sodium bicarbonate renders rapid loss of consciousness and death (4,6). As a result, the use of carbon dioxide liberated by chemical means will also be acceptable for the euthanasia of fish when compressed carbon dioxide gas cylinders cannot be used. Individuals who have demonstrated competency with this technique to qualified personnel (i.e. aquatic animal veterinarian or his/her designate) should perform the euthanasia of fish by carbon dioxide.
Sodium bicarbonate dosage: (30g/L or 120g/gallon)
Alka-Seltzer ® dosage: (1 tablet/ 20L or 2 tablets/ 10gallons)
Note: Fish euthanized with sodium bicarbonate or Alka-Seltzer® should not be used for human consumption.
Protocol: Fish should be placed into a container with an appropriate volume of water that will provide free movement of fish to be euthanized. Water should then be saturated with carbon dioxide from either a compressed gas cylinder (via the use of an air stone with a moderate flow rate for ~5-10 minutes) or by dissolving enough sodium bicarbonate or Alka-Seltzer® into the water (via the dosages previously provided). Fish should be removed only after ten minutes have passed since their last observed opercular movements (respiration) have occurred.
2007-04-26
01:57:49
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3 answers
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asked by
Grace
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Pets
➔ Fish