What is the purpose or reason behind the use of electrophoresis buffer?

Answer 1

The buffer conducts electricity and keeps the current from melting the gel as heat builds up. It is also a pH buffer to keep to DNA from degrading.

Tris-borate buffer (TBE) is advantageous for obtaining a higher resolution of smaller DNA fragments on agarose gels, when compared to the conventional tris-acetate buffer (TAE).

TAE buffer has its advantages over TBE buffer. TAE buffer can be stored for a long period of time without precipitation. But TAE buffer conducts higher electric current than TBE buffer, which will generate more heat and result in poor gel banding pattern.

Answer 2

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