VERY IMPORTANT SCIENTIFIC QUESTION!!! DNA,in classroom???

Question

What is the simplest way to extract the DNA from a human, in a classroom???
Please help.........or give links to good websites!!
Thank you!

Answer 1

I don't know about the DNA, but it's fairly easy to get a cell sample - just scrape a lollipop stick on the inside of your cheek. You can see the cells under a microscope.

Answer 2

Yes you can do a DNA extraction in the classroom. It will be good enough for the students to be able to see DNA but you can't really do anything with it beyond see that it is there and exists. My students did enjoy it. I found the lab information at this website

http://biology.arizona.edu/sciconn/lessons2/Vuturo/vuturo/dna.htm

All you need is gatorade or a mild salt solution. I used water and it worked. The students rinse their mouuths with the water/gatorade. add some soap solution then some isopropanol. The detergent will break open the cheek cells, the isopropanol will preciptate the DNA. (I worked in a research lab prior to teaching.) The students will be able to see a white strand, kind of looks like a hair that fell into their test tube but the hair is pure white.

The students do like it and it only takes about 15-20 minutes depending on whether the students focus and do the procedures or goof around.

have fun!

Answer 3

EDITING - PSSST! Give Scigeek93 the 'best answer' reward. That's a good link!

Do you want to just extract it, or do you need it pure? How much do you need? And ... I'd suggest using something other than human, you'll get into biohazard issues in the classroom. Get some E.coli K12 or B (harmless, biohazard I, unlike human!).
This is adapted from what I do in my research... It's the 'quick and dirty' method. Quick, but not very pure. It works on E.coli and on duck blood ... should work on human sample.

You'll need: centrifuge, micropipetters (1000 ul, 200 ul, 20 ul), tips, tubes, SDS 20%, TE, NaCl 5Mphenol, fume hood, (ideally), vortex, gloves, bunsen burner

If you've got a huge sample and are doing this as a demo, upscale it so you're doing it at 25 ml volumes... but I'd doubt you'd get that large a sample.

1)Pellet whatever cells you've got in a microfuge tube
2) resuspend in TE buffer (tris/EDTA)(I use 1.5 microliters)
3) add SDS to 1% (I use 25 microliters of 20% SDS) Vortex briefly. This lyses the cells.
4) Add phenol (fume hood!), roughly 500 microlitres. Shake, don't vortex, vigorously for a minute. Spin down (centrifuge).
5) remove aqueous layer (leave phenol and sludge), put into another tube. Add NaCl to 0.15 M (add roughly 15 microlitres of 5M NaCl)
6) add 1.5 volume ethanol, gently shake tube. You'll see strings or clumps of goo - looks a bit like snot
7) Using a pipet tip that's been sealed at the tip-end with a flame, fish out the goo (swirl the tip around, reel it on) (you can also spin it down)
8) Let the clump on your tip dry in a safe place without much air movement.
9) That clump is your DNA. Resuspend in whatever you need - TE or water. Don't use TE if you're going to do PCR.

Answer 4

You won't be able to extract the DNA in a school classroom, I doubt you'd have the equipment available. But yes cheek scraping would contain cells that contain DNA.

Answer 5

Not sure about extract but you can get some of the DNA from a piece of hair maybe.

Answer 6

You'd need to get alive cells, wherever they come from.
Sweat, saliva (the most common and reliable, and the one I would answer at a test), skin, blood, urine... anything you can think of. Hair is NOT very reliable: you'd need to get the follicle (the "root" of the hair: is the only part of the hair that contains living cells).
Then, you'd have to break the membrane of the cells (saporin is the most frequent method used... is like soap, really), and then treat the cells with DNAzol to extract it in "whole pieces".
Then, freeze at -20 or it will degrade straight away!

Answer 7

there's a very important site
http://science.nhmccd.edu/biol/bio1int.htm
DNA Genetics
The Biotechniques Virtual Laboratory University of Utah

Answer 8

Carolina biological and Bio-Rad both sell kits for making a "DNA Necklace" or DNA in a bottle...They're easy to do, using cheek scrapings.

Answer 9

In religious skill its that we are god's toddlers and he loves each and every people. In scientific skill i do no longer in all probability understand however the shown fact that people are so complicated and different than many different existence types and that we are waiting to have many emotions and emotions makes us different than for something of the species like reason, love, and so forth. animals are waiting to experience the countless thoughts yet no longer on the point of people. however the shown fact that people are waiting to do plenty, that's considerable, on condition which you're making it so. One is waiting to grow to be a great individual and impression the consequences of one in all those lot of issues. you could say that there is those that stay miserably properly even a number of them will beg for his or her lives. There fairly isn't one answer many will not be able to have their own perspectives.

Answer 10

we did a experiment in class about DNA's but we used a kiwi, so i don tknow how do extract a human DNA in class! lol.
i could have given you the method of the kiwi experiment but i dont have time.
you could maybe use a needle....