Explain the purpose of adding sodium hydroxide and copper sulfate In Biuret test.
NaOH:_____________________________
CuSo4:___________________________
2007-04-02 01:55:11 · 6 answers · asked by Unknown 1 in Science & Mathematics ➔ Chemistry
NaOH = to provide the alkaline solution.
CuSO4 = so that the copper (II) ions and peptide bonds react within the alkaline solution.
2007-04-02 02:08:20 · answer #1 · answered by noahukusa 2 · 0⤊ 1⤋
The biuret test is a reaction between copper ions and peptide bonds in an alkaline solution.
NaOH makes the solution alkaline
CuSO4 supplies the copper ions
2007-04-02 09:07:09 · answer #2 · answered by science teacher 7 · 0⤊ 1⤋
NaOH is a weak alkali, thus it is suitable 4 the test. CuSo4 bcos it reacts w/ the peptide bonds in the solution. Other than that, the only reason i can think of is that they're both in the Biuret reagent.
2007-04-02 09:11:30 · answer #3 · answered by shawn michaels pwns cena 4 · 0⤊ 1⤋
NaOH is used to denaturalize the protein,copper is bonded to peptide links and color is developed.
2007-04-05 22:14:36 · answer #4 · answered by Anonymous · 0⤊ 1⤋
Er...That is the exact question with the same formatting in my biology homework
2007-04-04 09:43:10 · answer #5 · answered by smileandclap 1 · 1⤊ 0⤋
Do you're own homework.
2007-04-02 09:04:49 · answer #6 · answered by bestonnet_00 7 · 0⤊ 1⤋