primer dimer in the amplified product?

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primer dimer in the amplified product?

2007-03-30 01:30:56 · 2 answers · asked by Rehan 1 in Science & Mathematics ➔ Biology

2 answers

Primer dimers form because of some level of complimentarity between the two primers in PCR. You can avoid them either by redesigning the primers. You might also be able to raise the temperature of primer annealing a few degrees and prevent them.

2007-03-30 01:56:01 · answer #1 · answered by hcbiochem 7 · 0⤊ 0⤋

Here is the answer:

http://www.youtube.com/watch?v=dnmZFOADwI8

2013-11-10 10:34:42 · answer #2 · answered by Anonymous · 0⤊ 0⤋