2007-03-28 23:32:21 · 4 answers · asked by Anonymous in Science & Mathematics ➔ Biology
You need to pick (in a sterile manner) some of the bacteria spread from the first agar plate and streak them onto a new agar plate, taking care of turning the plate of a few degrees every so often. To be sure that you are in limiting conditions, which will allow you to end up with individual colonies, you could streak the bacteria a second and third time on sequential plates - therefore performing a "serial dilution" of your bacterial titer. So at least in one of them you will have few enough bacteria to grow sparse and make individual colonies.
You may find a diagram on how it is done here:
http://loudoun.nv.cc.va.us/vetonline/vet132/micro/isolation_streak_diagram.gif
2007-03-29 01:09:28 · answer #1 · answered by Jesus is my Savior 7 · 1⤊ 0⤋
Subculture Bacteria
2016-12-10 14:36:58 · answer #2 · answered by seeley 4 · 0⤊ 0⤋
Use a sterile loop to get a sample from the bacteria and streak this sample on to another sterile petri plate.
2007-03-28 23:39:54 · answer #3 · answered by physandchemteach 7 · 0⤊ 0⤋
well, according to the USP, Nutrient Agar is not a selective or indicative media. Anyway it is normally used to culture bacteria, but not fungi..
2016-03-18 06:10:21 · answer #4 · answered by Anonymous · 0⤊ 0⤋