Is there an ideal antibiotic resistant gene used for stable or transient expressions?

Question

antibiotics are able to select competent cells that contain the antibiotic resistant gene. But is any antibiotic special to give a stable or transient expression for the cells after tranfection?

Answer 1

for e. coli, ampicillin is the standard and most well-tested antibiotic for transformation with plasmid. carbenicillin is similar, but it does not degrade as rapidly as ampicillin. in my experience kanamycin tends to kill some of the bacteria that have the plasmid, i.e. the selection is tighter.

for human (and probably mammalian cells in general), neomycin is the gold standard. it tolerates a wider ranger of expression of the resistance gene than hygromycin or puromycin, which are the other two that are commonly used. this is important when you have a complicated design, e.g. an internal ribosome entry site driving the resistance gene. if not, all are acceptable. as far as the different between stable and transient, it might depend on what you're trying to do. for my transients, i don't have a selection so all i have in there is the streptomycin to kill the bacteria and yeast and whatnot.

you have to keep in mind that T-antigen and EBNA-1 have been integrated into various lines using neo, puro, or hygro, so if you want to use one of those lines you cannot select using that antibiotic. otherwise the cells will lose the plasmid.

Answer 2

The older antibiotics paintings purely besides as they ever did. The "germ-resistant" extra cutting-edge antibiotics are a rip-off to get medical doctors to prescribe extra costly drugs so the huge Pharmaceutical industry can earn extra funds. whilst your well being care provider prescribes an antibiotic tell him which you fairly prefer a useful one. They paintings purely besides and fee plenty much less. as far as MRSA is worried it involves semi-man made antibiotics that have been printed as ineffective for gram-destructive micro organism as early as 1963. on condition that they have been by no capacity helpful against those micro organism that have been around on condition that earlier 1949 how can every person say that the antibiotic grew to alter into ineffective. It exchange into by no capacity helpful. There are at the instant circumstances of antibiotic resistant lines of tuberculosis. Such issues take place whilst infections are taken care of with antibiotics yet no longer long sufficient to kill the full an infection. a lot of those circumstances have arisin in prisons (somewhat in Russia) by using fact the the prisoners are taken care of untill indications subside and then taken off of scientific care, or many times they are released earlier the scientific care is comprehensive and the exconvicts won't be able to handle to pay for to proceed the scientific care.