what is primer dimer? how to over come this problem?

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what is primer dimer? how to over come this problem?

2007-03-26 13:09:51 · 2 answers · asked by teck kim 2 in Science & Mathematics ➔ Biology

2 answers

Primer dimers form in PCR reactions when the two primers used are partially complimentary to each other. The only way to completely avoid them is to make different primers that have no complementarity to each other. You might also try a higher temperature for the annealing stage of PCR.

2007-03-26 13:19:22 · answer #1 · answered by hcbiochem 7 · 1⤊ 0⤋

Here is the answer:

http://www.youtube.com/watch?v=dnmZFOADwI8

2013-11-10 18:21:38 · answer #2 · answered by Anonymous · 0⤊ 0⤋