<< Genetics Questions >>> DNA FINGERPRINTING T0T;;?

Question

1. What caused the DNA to become fragmented?

2, WHat determines where a restriction endonuclease will 'cut' a DNA molecule?

3. A restriction endonuclease cuts 2 DNA molecules at the same location, What can I assume is identical about the molecules at the location?

5. if an individual has a nutrient deficiency due to poor diet and is missing a particular amino acid, then Which process of protein synthesis would be more affected??

plz!~~

Answer 1

I'm not sure what the experiment you did was, but here are my answers.

1. The DNA is fragmented because of the cutting at certain locations due to the endonucleases.

2. Restriction endonucleases cut DNA molecules at a specific sequence that is recognized for that specific endonuclease. It will not cut unless there is that specific nucleotide sequence present.

3. If the endonuclease cuts the DNA molecule at 2 different location, you can assume that the sequence is the same at those locations. Therefore, we can also agree that the endonuclease is specific for that sequence and if you know what the endonuclease is for , then you know what the sequence is at that spot.

5. Well, transcription is going from DNA to RNA , and therefore does not involve the amino acids as of yet. However, when translation occurs on the ribosome where RNA is used to create a protein, a missing amino acid can have an adverse affect because the protein will not be able to be completed because the amino acid is not present. Therefore, the process most affected is translation.

Good luck and hope this helps!