can someone tell me about okazaki fragment......?

Question

actually im doing my assignment about DNA replication.i know about DNA polymerase, leading and lagging strand.but i need more information about okazaki fragment.

Answer 1

It is difficult to explain without knowing how much you know about DNA replication.

Try http://www.answers.com/okazaki%20fragment?ff=1 for some info.

If you update your question and let me know, roughly what you know about DNA replication I will be able to help you a bit more. Do you know what is meant by leading and lagging strands?

Answer 2

So you know about DNA polymerase and leading and lagging strand, so I assume you understand how the new DNA is manufactured and the fact that it is extended at the 3' end. Since DNA strands are antiparallel, the one strand being read from 3' to 5' and synthesized from 5' to 3' has no problem. However, the lagging strand is being read from 5' to 3'. Since the new strand can't be synthesized from 3' to 5', the result is okazaki fragments, that is, primase is used to create RNA primers in certain segments, crreating some single strand DNA and some DNA/RNA hybrid. The polymerase then uses the primer's 3' end as a means to extend and code for the area between RNA primers. These segments of DNA are the Okazaki fragments, and later on in the process other enzymes remove the RNA segments and use the 3' end of the Okazaki fragments to fill in those gaps, creating a fully formed DNA strand.

Answer 3

These are short of DNA with an RNA primer at the 5' terminus created on the lagging strand during DNA replication. It was discovered in 1968 by Okazaki(s).

Answer 4

I can tell you about Okazaki as I live near there and go shopping at Aeon somtimes. I don't get what you mean by Okazaki fragment though.