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Question

salting in ,salting out dialysis.
it is important please

i need detailed information on it
any website address will be more helpfull to me


but any way i thank who
gave the answer
trying to give answer

i need detailed information on it
any website address will be more helpful to me


but any way i thank who
gave the answer
trying to give answer

Answer 1

Salting in is a solubilization of a protein in water by addition of a small amount of a salt. The small amount of salt helps stabilize charge relationships in the protein.

One can precipitate a protein by adding a high concentration of a salt. This is salting out.

Once one has salted out a protein, dialysis can be used to free the protein from, the salt. Dialysis uses a semipermeable membrane (cellophane comes to mind) such that pure water diffuses across the membrane to try to dilute the salt. Protein molecules cannot cross the membrane, because they are too large.