Does Biuret's solution bond to protein molecules?

Question

In a test tube where the Biuret's reagent solution is present, it appears as though the reagent bonds with yeast cells that are also present in the test tube. I am asking this question because I want to directly (through scientific experimentation) determine if the cell membrane of yeast cells contains protein (for a biology class). Does Biuret's solution bond with protein molecules?

Answer 1

Well, my friend, first of all, Biuret test is a confirmatory test for proteins. It recognizes peptide bonds or amide type linkages (CO-NH bond). The reagent is actually a mixture of copper sulphate solution and sodium hydroxide solution and the violet or lavender colour obtained is due to a complex formation with Cu has the central atom and the ligands will be N-donor ones of the protein fragments. The cell wall of yeast contains chitin if I recall, which a nitrogen containing polysaccharides and cell membranes of all cells contain some amount of proteins but contain a larger proportion of phospholipids.

Answer 2

The biuret reaction indicates the prescience of a few peptide bonds; it is not definitive for protein or polypeptides.

Yes the cell membrane of yeast cells contains protein and any reagent such as "ninhydrin" which reacts with Amino acids should detect it with (in the case of ninhydrin) production of a purple color If you are not familiar with Ninhydrin look it up on Yahoo! search or google.

Answer 3

Biuret is a condensation compound of urea, reminiscent of two molecules of urea much less certainly one of ammonia. it could additionally stick to to the smart group and sophistication of organic and organic compounds with the final shape RHN-CO-NR'-CO-NHR" wherein R, R' and R" are organic and organic residues. The biuret attempt a chemical attempt for proteins and polypeptides. it fairly relies on the biuret reagent, a blue answer that turns violet while it fairly is provided in touch with protein, or any substance with peptide bonds. The attempt and reagent do not fairly comprise biuret; they're so named because of the fact the two biuret and proteins have the comparable reaction to the attempt. i'm hoping that's what you meant, because of the fact it form of feels from the pronounciation which you will additionally recommend 'burette', this is an tool in lots of cases utilized in titrations to dispense precise volumes of liquid

Answer 4

yes