what is the reason for the antibiotic resistance gene?

Question

what is the reason if it cancels out the effect of antibiotics.
extra info: this is for genetic engineering in biology

how can scientists take adavantage of it?

Answer 1

Well what commonly happens is that some mutation lead to a gene that codes for a protein that confers resistance to a certain antibiotic. There are several ways this can happen; one way it can happen is that it codes for an enzyme which breaks down the antibiotic, such as the ampicillin or tetracycline resistance genes, or it can be a change in the structure of the protein or structure the antibiotic affects. In any case, it makes any bacterium with the gene immune to the antibiotic.

This is very useful in genetic engineering because what it can do is verify genetic transformation of bacteria. Let's say we want a certain gene to be transformed into bacteria in a plasmid. What you can do is place that gene on a plasmid that also confers antiobiotic resistance, such as an amp plasmid, conferring resistance to ampicillin. Then you mix the plasmid and bacteria together, do something to allow the plasmid to be incorporated into the DNA, and then incubate the bacteria on a culture medium containing the antiobiotic. What will happen is that all the bacteria without the ampicillin resistance plasmid and hence the gene you want in them will die, and you are left with several colonies, all containing the gene you want.

Answer 2

Antibiotic resistance in bacteria happens when a bacterial gene that otherwise is unable to metabolize the antibiotic compund (meant to kill them), mutate to do so, rendering it ineffective, and allowing the bacteria to survive. It also happens that certain bacteria acquire resistance through plasmids (small circular DNA molecules that are self replicative and are independent of the bacterial chromosome) from other resistant organisms. Plasmids contain antibiotic resistant genes.

Answer 3

Antibiotic resistance is found in the bacteria, not in the organisms who take antibiotics. It is there because it works in benefit to the organism it is found in (the bacteria) by allowing it to survive even when faced w/ antibiotics.

It cancels out the effect of antibiotics and that's why its able to survive. It enables the further proliferation of the bacteria. Yay natural selection!

Answer 4

Well 4 1 they are different so they need different things then we do so that is why they have a resistance from some antibiotics

Answer 5

Natural Selection.