When innoculating agar plate, Is streaking the plate same as isolation technique? Please clarify.?

Answer 1

Cultures ordered from a supply company or stock center will probably not consist of genetically identical bacteria. The bacteria will all be of the same species, and available as a single strain. However, random mutations may still exist due to the large number of bacteria present. To obtain a source of genetically identical bacteria, streak plates are used. Streaking a plate allows the bacteria to be spread out so that a single bacterium can be isolated from all other bacteria. This technique is called streaking for individual colonies. Since bacteria are so small, you will not be able to see that isolated bacterium. However, that bacterium will reproduce itself by binary fission (typical division time is on the order of 20 minutes), resulting in bacteria which are genetically identical to the original bacterium and to each other. These bacteria are visible as a small round colony growing where there had been one isolated bacterium. This method allows you to use the individual colony repeatedly and expect similar results.

Answer 2

Yes in a way - through subsequent streaks around the agar plate you are isolating the bacteria - using differential or selective media will further enhance these colonies!

Answer 3

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Answer 4

well, when you innoculate a certain colony and transfer it into other agar plate that is the time you isolate that certain colony.