hey, bio pros out there. What are the use of genomic dna and plasmid of E.coli?? thx every one..?

Answer 1

I went on the web site for you and found exactly what you are looking for. just type in genomic DNA and plasmid of e-coli and it gives you all the information you need. It says how they take purified DNA and mixed it with E-coli and grew a colony. Go on "search the Web" and it's there. Hope I gave you some help.

Answer 2

If you wanted to introduce a gene that isn't present naturally in E.coli into it's genomic dna (total natural dna), you would use a plasmid with the gene you are introducing already in it. You introduce the pasmid to the E.coli and the E.coli takes it up and encorporates into it's genomic dna (total dna). The purpose of this is to: 1) show that your plasmid works and 2) E.coli reproduces very well and will produce a large amount of the gene you want. You can then lyse (desentigrate) the plasma membrane of E.coli and get the protein (made from the DNA you incorporated from the plasmid). You can then sequence this protein and make an antibody for it, or do whatever you wish. with it.