Describe DNA replication using terms DNA polymease, Okasaki fragments, leading and lagging strand & DNA Ligase

Question

Hey Everyone! Biology Help Please! Ah..We are currently learning about DNA and I'm lost! I have till Thurs. to do this! I can't seem to find a good picture or explanation. Our Biology Book does not explain DNA in detail! I would like to know: How does DNA Replication occur or work? Steps?...And how do the terms DNA Polymerase, Okasaki Fragments, Leading and Lagging strand and DNA Ligase come into play with DNA Replication! Thanks:)

Answer 1

I have included a link to a movie that you should watch.
DNA replication begins with the "unzipping" of the parent molecule as the hydrogen bonds between the base pairs are broken.
Once exposed, the sequence of bases on each of the separated strands serves as a template to guide the insertion of a complementary set of bases on the strand being synthesized.
The new strands are assembled from deoxynucleoside triphosphates.
Each incoming nucleotide is covalently linked to the "free" 3' carbon atom on the pentose (figure) as
the second and third phosphates are removed together as a molecule of pyrophosphate (PPi).
The nucleotides are assembled in the order that complements the order of bases on the strand serving as the template.
Thus each C on the template guides the insertion of a G on the new strand, each G a C, and so on.
When the process is complete, two DNA molecules have been formed identical to each other and to the parent molecule.

A portion of the double helix is unwound by a helicase.
A molecule of a DNA polymerase binds to one strand of the DNA and begins moving along it in the 3' to 5' direction, using it as a template for assembling a leading strand of nucleotides and reforming a double helix. In eukaryotes, this molecule is called DNA polymerase delta (δ).
Because DNA synthesis can only occur 5' to 3', a molecule of a second type of DNA polymerase (epsilon, ε, in eukaryotes) binds to the other template strand as the double helix opens. This molecule must synthesize discontinuous segments of polynucleotides (called Okazaki fragments). Another enzyme, DNA ligase I then stitches these together into the lagging strand.

Answer 2

hmmm.. the leading strand is called leading because it is 1st to finish without having any problem... so it works on 5'3' then the strand goes downwards opposite to its strand.. it must be 3'5'... while the lagging strand is the 2nd strand that should go downwards opposite to the 2nd strand.. it will be working on the strand 3'5'. so the strand would be created as 5'3' with fragments which aren't completed.. thats why it makes okazaki fragments! there is only one helicase!