This is an experiment. Please help me if you know the answer.?

Question

We have added starch to pancreatin. You know that pancreatin contains several enzymes: amylase, lipase. trypsin and chymotrypsin, and nucleases. So basically, it'll breakdown starch into maltose. Then we added benedict reagent and heated it for about 5 minutes. It turned dark green solution! There is no brick red precipitate as we've all expected. What could be possibly the reason behind this? Thanks :)

Answer 1

Pancreatin is the mixture of enzymes obtained by extraction of the pancreas and consisting essentially of lipases, amylase and proteases. Pancreatin hydrolyses fats, changes protein into proteases and derived substances and converts starches into dextrin’s and sugars.
Well, if u perform benedict’s test with starch there will be no change in color on addition of benedicts reagent, whereas when performed with dextrin there is appearance of green color and this is due to the fact that dextrin’s have more reducing activity than starch. Dextrins are intermediate products obtained during the course of hydrolysis of starch by the action of amylases.

So the possible reason is partial hydrolysis of starch resulted in formation of dextrin’s which on treatment with Benedict’s reagent gave a green color.

To confirm its dextrin u may perform the iodine reaction (red/violet/purple color)

Answer 2

as you shoul know benedict test is also know as the semi qualitative
if there is a change in colour from blue to yellow to green to orange to red and then brick-red it means that there is presence of reducing sugars
in your experiment there is not enough redocing sugar for the colour to completly change to red