2007-03-02 15:07:45 · 3 answers · asked by melissa 1 in Science & Mathematics ➔ Biology
The purpose of this is so that you can see the plasmid has been transfected by looking at the cells that light up. Cells that don't light up, did not get the plasmid, and thus do not get the bioluminescent gene and more importantly the gene of interest that you intended to transfect.
2007-03-02 15:33:26 · answer #1 · answered by jason e 2 · 0⤊ 0⤋
If you tag a gene of interest on the plasmid with a bio luminescent gene like GFP (Green Fluorescent Protein) so that GFP is in frame with the gene of interest, AND place the fusion gene under some sort of expression control in the way of a promoter, you can stick the engineered plasmid into a cell and get green fluorescence at the location where the gene of interest is being expressed.
2007-03-02 23:25:35 · answer #2 · answered by BP 7 · 0⤊ 0⤋
the gene in question would be easier to track...just look for the light, so to speak.
2007-03-03 17:41:01 · answer #3 · answered by bad guppy 5 · 0⤊ 0⤋