what the factors influence the supercoid DNA run faster than linear and nicked in gel?

2007-03-01 21:50:05 · 1 answers · asked by teck kim 2 in Science & Mathematics ➔ Biology

1 answers

Supercoiled plasmid DNA runs faster in gel because it is a highly compact conformation whereas linear and nicked DNA are not (they are pretty extended so it is harder for them to migrate through the agarose pores)

2007-03-01 21:57:09 · answer #1 · answered by ger 3 · 1⤊ 0⤋

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