little bio help dna?

Question

what do gene gun heat shock and electroporation have in common and how they are diffrent

Answer 1

They are all methods of introducing DNA into a cell, they all "shock" the cell in some manner, the gun, physically wounds the cell, the heat shock with heat, and electroporation with electric shock.

The Gene gun is mechanical method that bombards the cell with tiny particles coated with your DNA, this is most successful in plant tissues and tissue culturing.

Heat shock is typically used in bacterial transformation, involves a plasmid and competent cells and is efficient for transformed bacterial colonies. No special equipment is required, this is a long-standing tried and true method.

Electroporation is used in both plant protoplasts and bacteria, is a more effecient method in bacteria than heat shock, requires a different type of competent cell. This method requires cuvettes and an electroporator that delivers the electric shock.

Answer 2

Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance into a cell, such as loading it with a molecular probe, a drug that can change the cell's function, or a piece of coding DNA.

This is something very cool..do check out…..

http://www.hort.purdue.edu/hort/courses/HORT250/animations/Gene%20Gun%20Animation/Genegun1.html

in case of electroporation the gene transfer is mediated by altering the permeability of cell membrane by inducing electrical potential…whereas in gene gun…the DNA is coated with heavy metal such as tungsten or gold and at a high velocity is bombarded into the target cell. Electroporation involves electric potential and gene gun involves high velocity bombardment.

Both these methods are employed for transfer of gene or DNA into target cells.

Answer 3

E. they're unwound. Complimentary base pairs then align against the template strand (they don't covalently bond), to offer an almost-replica of the coding strand (U is used as a replace of T). DNA helicase is what unzips the DNA. One strand is used interior the DNA, the different is a sparkling strand.

Answer 4

I have no idea what you are talking about. Sorry, I was just curious to check your question.

Answer 5

i dont know anything about them...
these links could be useful....

http://en.wikipedia.org/wiki/Electroporation
http://en.wikipedia.org/wiki/Gene_gun