can someone help me with biology???

Question

can somebody give me a sentence for gel electrophoresis

Answer 1

A technique for resolving a mixture of macromolecules. A sample is put in the gel (which act as a molecular sieve), then an electrical field is applied that pulls the macromolecules down the gel. The macromolecules (DNA or protein) migrate through the pores of the gel at different rates, so the mixture is resolved according to mass, shape and charge. I doing a gel right now!!!

Answer 2

The police had to await the gel electrophoresis results as to ascertain if the DNA sample was that of the Suspect in custody

Answer 3

it's a method of DNA analysis, where you measure the distance between certain codes..



here's wikipedia's version:
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point. Gel electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or immuno-blotting for further characterization.

In the case of nucleic acids, the direction of migration, from negative to positive electrodes, is due to the natural negative charge carried on their sugar-phosphate backbone. Double-stranded DNA fragments naturally behave as long rods, so their migration through the gel is relative to their radius of gyration, or, for non-cyclic fragments, roughly size. Single-stranded DNA or RNA tend to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as sodium hydroxide or formamide, are used to renature the nucleic acids and cause them to behave as long rods again.