Amplification via bacteria and PCR?

Question

I am working on this particular project but I do not really understand the following.

I am supposed to clone an N and a C terminus of GFP protein into a particular host cell. I am given those 2 plasmids and am asked to grow in bacteria first and then amplify it through PCR.

I am just wondering why shouldn't I just amplify it by either PCR or bacteria. I know both have its pros and cons but why do you have to amplify it in 2 different ways? Wouldn't you be losing money on that?

I do not want the plasmid at the end of the day. Why do I still need to transfect with bacteria then?

Answer 1

Grow the bacteria to amplify the plasmid. PCR is amplifying the target genes that you want i suppose.