How do you extract DNA from leaves and stuff?

Question

I need to perform DNA extraction on leaves or meat. How can i do it?
I tried this website: http://learn.genetics.utah.edu/units/activities/extraction/
but it didnt work. I followed everything as it is there. So why is it wrong? I can only get waste proteins precipitated out at the interface between the ethanol and SDS/blended pea mix.

Answer 1

put your leaves in a blender and mix them till you get a mushy thingy then you pour that in a glass and add 50ml of puffer ( water:detergent(the one you use for dishes)=9:1 and 2%kitchen salt (w/v))
cook it on 60 degrees celsius for 15 minutes and use a straw or something like this to mix it every now and then
pour this through a coffee filter and what goes through the filter collect in an epruvette
cool it on ice
ps sorry for misspelings
add 0,75ml of annanas juice (it has bromelin which will help with isolating DNA from proteins)
incubate for 10 min on 36 celzius
now slowly pour same amount of ice cold 96% etanol on the edge of the epruvete (so they don`t mix)
put all that on ice
after a while you will be able to wrap DNA on a wooden tip
DNA will look like a thin white line
we did it in my colleg and it really works

Answer 2

Materials:

* 55oC water bath
* 200 ml beaker
* Glass stirring rod or wooden toothpicks
* 10 ml graduated cylinder
* Mortar and Pestle
* Ice bucket/Ice
* Plant DNA source
o 1.5 g raw wheat germ or
o 3 g dry lentil beans or
o 6 g fresh peas (not canned) or
o 9 g onion
* 6 g Adolph's 100% Natural tenderizer (unseasoned)-must contain papain
* 10 ml Palmolive dishwashing liquid (clear, concentrated)
* 20 ml ethyl alcohol or isopropyl alcohol (chilled on ice)
* Baking soda solution pH 8.0
o 8.4 grams sodium bicarbonate (baking soda) in 100 ml tap water

Procedure:

1. Place 3 g of lentils into 100 ml of H20 and allow them to soak in the hot water bath for at least 1 hour. (the lentils could be soaked prior to class).
2. Thoroughly crush the lentils with the mortar and pestle.
3. Add 10 ml of the Palmolive liquid and stir briefly. Allow this mixture to soak in the hot water bath for 5 minutes. Do not allow the temperature to exceed 60oC as this may denature the DNA.
4. Add 6 g of the meat tenderizer and 20 ml of the baking soda solution to this solution and continue to allow it to soak in the hot water bath at 55oC for an additional 10 minutes.
5. Take the beaker out of the hot water bath.
6. Strain the mixture through a fine nylon mesh or piece of cheesecloth to remove any plant debris.
7. Cool the extract on ice for 15 minutes.
8. Gently pour 20 ml of ethanol into the beaker, trying not to mix it with the solution. You want the alcohol to form a layer on top of the detergent-treated cell solution.
The cell debris layer is more dense and will remain on the bottom of the beaker. The DNA is less dense than the cell debris but more dense than the alcohol. Therefore, it will remain where the two layers meet. Since the DNA is not soluble in alcohol, it comes out of solution (precipitates)
9. Observe the DNA as a stringlike or "snotty" substance at the interface of the two layers. Proteins associated with the DNA may make it appear white.
10. Use a toothpick or glass rod to slowly spool the material at the interface of the two layers (not in the lower layer). Do not dip into the bottom or you will disturb the lentil waste. You should be able to now see some DNA.
11. The DNA can be saved for more experiments by storing it in a sealed container in the freezer. removing it from the small glass container.

Answer 3

Leaves don't have dna, only people do. Duh!