2007-02-22 00:19:05 · 2 answers · asked by aljon l 1 in Science & Mathematics ➔ Chemistry
For paper and thin layer chromatography (TLC) the Rf values are used to determine if the "spots" of the compounds have traveled the same distance and therefore might be the same as one of the standards.
The Rf value is also called the retardation factor (in planar chromatography). It is the ratio of the distance travelled by the center of the spot to the distance simultaneously travelled by the mobile phase.
Rf = Known or Unknown/Solvent front.
By definition the Rf values are always less than unity. They are usually given to two decimal places.
2007-02-22 00:49:42 · answer #1 · answered by Richard 7 · 8⤊ 0⤋
I assume u know the meaning of Rf values. Its application? To identify a particular chemical substance. It is like a 'fingerprint' to identify an unknown substance based on known Rf value of a list of substance.
2007-02-22 00:55:38 · answer #2 · answered by Anonymous · 0⤊ 1⤋