2007-02-19 19:40:07 · 3 answers · asked by abisiboy 1 in Science & Mathematics ➔ Biology
Simply is the pH at which the charge of a protein is 0, i.e. when the negative charges of the R-chains of the aminoacids balance the positive charges.
Below the isoelectric point proteins carry a net positive charge, above it a net negative charge. Due to a preponderance of weakly acid residues in almost all proteins, they are nearly all negatively charged at neutral pH. The isoelectric point is of significance in protein purification because it is the pH at which solubility is often minimal and at which mobility in an electrofocusing system is zero (and therefore the point at which the protein will accumulate).
2007-02-19 19:57:14 · answer #1 · answered by Jesus is my Savior 7 · 1⤊ 0⤋
Isoelectric Point Of A Protein
2016-11-01 08:39:46 · answer #2 · answered by ? 4 · 0⤊ 0⤋
the pH at which the protein has no charge
2007-02-23 18:43:14 · answer #3 · answered by BP 7 · 0⤊ 0⤋