Can someone help me out with coding strands of DNA??

Question

Ok. I missed class last week due to personal reasons. Anyways, I missed the section on how to code strands of DNA. I go to OSU, so of course my professor wouldnt be able to help me since theres over 400 people in the class and I don't know anyone else taking this class.

Anyways, we got a worksheet to do with 3 questions. I read in my book on how to do this, but I don't understand it.

The questions we got were:

The following nucleotide sequence is the coding strand of DNA:
ATG ACT TGT GTC CTA TAG

Please provide the sequence of the template strand.

Please transcribe the mRNA from this DNA.

Please translate the amino acid sequence from this message.


Can anyone help me out? And perhaps, give me a short summary on how to do this?

Answer 1

WHY do people get these things mixed

Nucleic acid sequences are always written in the 5'->3' direction

so you have the coding sequence:

5' -ATG ACT TGT GTC CTA TAG -3'

The template sequence you can find easily by pairing A with T, T with A, G with C and C with G

5' -ATG ACT TGT GTC CTA TAG -3'
3' -TAC TGA ACA CAG GAT ATC -5'

Now, for a not very advanced class the professor would be content with the answer TAC TGA ACA CAG GAT ATC as the template sequence. However that would be wrong, since nucleic acid sequences have to be written in the 5'->3' direction so the truly correct answer is:
5'-CTA TAG GAC ACA AGT CAT-3'

mRNA has the same sequence as the coding DNA with only difference that you have U instead of T:
5' -AUG ACU UGU GUC CUA UAG -3'

You need a table with the code (every 3 bases make-up a codon that codes for a specific amino acid). You can also use on-line tools like: http://au.expasy.org/tools/dna.html...
M T C V L Stop or if you prefer 3-letter code

Met Thr Cys Val Leu stop (well stop is not part of the protein but its just to show that the last codon is a stop codon)

Answer 2

There are 4 bases in DNA : A, T, C, G.
A always goes with T and C always goes with G
There are 4 bases in RNA: A, U, C, G
A always goes with U and C always goes with G

The question gives you a coding strand and from this a template strand must be made. With the above rules this means that the template strand must be:
TAC TGA ACA CAG GAT ATC

From the template strand a mRNA is transcribed and with the above rules the mRNA strand must be:
AUG ACU UGU GUC CUA UAG

From the mRNA strand a sequence of amino acids can be deciphered. Each 3 bases = 1 codon. each codon = 1 amino acid. You should have been given an amino acid/codon table or something. Use the mRNA strand to figure out the amino acid sequence.

Good luck!

Answer 3

DNA has 2 strands. both strands are the different of one yet another so that they tournament like a puzzle. A promoter is the very similar and is the different of the area of DNA it needs to began coding. it is going to in trouble-free terms bind to the area it is its finished opposite. So if the promoter is GGGCCC it is going to in trouble-free terms bind to DNA it is CCCGGG. it basically isn't waiting to bind to at least some thing else.

Answer 4

I could be wrong, but I think the amino acid sequence as translated from the mRNA would be:
(Start) Methionine, threonine, cysteine, valine, leucine, stop codon

Answer 5

the mRNA will be UAC UGA ACA CAG GAU AUG
and the amino acids in order are:tyrosine,stop codon,threonin,glutamine,acid aspartic,start codon
and the template strand is TAC TGA ACA CAG GAT ATC

Answer 6

TAC TGA ACA CAG GAT ATC - template strand
UAC UGA ACA CAG GAU AUC - mrna

i dont have the amino acid sequence chart with me at the moment..