2007-02-18 07:00:05 · 3 answers · asked by mylinhle86 1 in Science & Mathematics ➔ Biology
Gene Wilder
2007-02-18 07:04:32 · answer #1 · answered by michelle 5 · 0⤊ 0⤋
Lots of miRNAs never make it in, and also if a gene is not expressed in the tissue you collected your RNA from then it will not be in your cDNA library. Also genes that are highly regulated at the level of mRNA stability via degredation. Really the extent of coverage for a cDNA library depends first on how you created the cDNA library. Some transcribed mRNAs do not prime well for RT with the poly d(T) method so if you made your library like this most poly (A) mRNAs would show up but not all. Genes with short polyA stretches or those mRNAs that have had their's degraded are an example, and this can be many genes depending on the conditions and cell type.
2007-02-18 16:13:36 · answer #2 · answered by rgomezam 3 · 0⤊ 1⤋
Posh! Silly Human! How could that not be in your knowledge?!? I, being a Norwegian top of the line scientist, that has created a mechine that can take you back in time (Norwegian has not decided to share my creations with anybody else) allow you to talk to animals and various other creations which have left Norwegian brilliant and the US a mere memory...
-Your Scientist
Bubba Johansson
2007-02-18 15:16:03 · answer #3 · answered by I'm not strange, just unique 2 · 0⤊ 2⤋