My question is to know how it is occur immunofluorescence analysis of the Intermediate Filament cytoskeleton in U373 MG Cells, plese give more details about it
2007-02-17 02:13:05 · 5 answers · asked by Jugux A 1 in Science & Mathematics ➔ Biology
Epithelial cells contain desmosomes, special intercellular junctions providing sites of membrane attachment for intermediate-sized filaments of the cytokeratin type (tonofilaments). Such sites of anchorage of tonofilaments appear as dense plaques on the cytoplasmic side of the desmosomal membrane. We have isolated desmosome-enriched fractions from bovine snout epidermis and tongue mucosa and have characterized the major protein associated with the desmosomal plaque. This protein occurs in equimolar amounts of two polypeptides of Mr 250,000 (desmoplakin I) and Mr 215,000 (desmoplakin II) which are chemically and immunologically related. Antibodies raised against desmoplakins allow the identification and localization of this protein in epithelial cells grown in tissues or in vitro and show crossreaction in species as diverse as man, mouse, and chicken. Using immunolocalization at the light and electron microscope levels, we show that these antibodies bind specifically to desmosomal plaques. Antibodies to desmoplakins have been used successfully for detection of desmosomal proteins in a broad variety of epithelium-derived human tumors, including primary carcinomas and their metastases, irrespective of the morphology of the specific tumor. Nonepithelial tumors examined have been negative. We propose to use antibodies to desmoplakins and to cytokeratins in pathological diagnosis as two independent markers for the positive immunocytochemical identification and classification of epithelium derived tumors.
2007-02-17 02:17:39 · answer #1 · answered by BARROWMAN 6 · 0⤊ 0⤋
As a microbiologist, I am interested in your question.
Could you refine it? I am not sure if I can help you but I try to do my best.
2007-02-17 16:34:58 · answer #2 · answered by silberstein_9 3 · 0⤊ 0⤋
Blimey, that barrowman knows his stuff ! !
2007-02-17 10:22:26 · answer #3 · answered by iain xx 4 · 0⤊ 0⤋
if you wanna know all that, go to friggin college.
2007-02-17 10:24:20 · answer #4 · answered by pamela g 4 · 0⤊ 0⤋
What a stupid question...Not funny
2007-02-17 10:16:29 · answer #5 · answered by Anonymous · 0⤊ 1⤋