plant cytogenetics-squash method?

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plant cytogenetics-squash method?

I need a protocol for the preparation of plant chromosomes using the squash method. do u have one?

2007-02-16 07:35:22 · 1 answers · asked by saleh_11j 2 in Science & Mathematics ➔ Biology

1 answers

I'd love to help you out, but I don't know one. On the other hand a GREAT protocol for viewing plant chromosomes is a nitrous oxide method developed by Akio Kato in Jim Birchler's lab from the University of Missouri. check out Akio Kato's papers from pubmed for the protocol. I went from viewing 50 slides for a good spread to viewing just one and getting lots of good cells. JC Lamb also has a lot of papers out using that same method.

2007-02-16 08:17:03 · answer #1 · answered by btpage0630 5 · 0⤊ 0⤋