Hi I really need help with serial dilutions, I just dont understand it, I've checked out several websites and it doesnt make sense. Could someone expain it STEP by STEP using an example and not just posting a link to a website thank you!
2007-02-15 02:33:09 · 4 answers · asked by deus82 3 in Science & Mathematics ➔ Chemistry
Lets say you want a solution of a final concentration of 0.01 mg/mL. Weigh your material, lets say 10mg, into a 100mL volumetric flask. Dilute to the mark, this will be 0.1 mg/mL concentration, and we can call it solution A.
Mix solution A well (so your solution is homogeneous), then pipet 10mL from the flask and place it into a 100mL volumetric. Dilute to the mark, and you will have 0.01 mg/mL.
As an equation, you would see it like this:
(10mg/100mL) x (10mL/100mL) = 0.01 mg/mL
Cancel your units, and you get mg/mL
Hope this helps.
2007-02-22 09:27:07 · answer #1 · answered by purdue_chemist01 1 · 0⤊ 0⤋
you make your first dilution to the concentration you want it. let it sit for a bit (let's call this dilution #1) and then take a given amount from this vial and add it to another. add the solvent you're diluting it in and let the 2 mix (dilution #2).
i let mine sit for a few moments between dilutions
then all you have to do is the same dilution using 2 to get your 3rd solution.
2007-02-15 03:43:48 · answer #2 · answered by shiara_blade 6 · 0⤊ 0⤋
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2016-12-04 05:14:07 · answer #3 · answered by ? 4 · 0⤊ 0⤋
check this website...
http://www.woodrow.org/teachers/esi/2002/Biology/Projects/lab_skills/ls8/
2007-02-22 05:58:25 · answer #4 · answered by hsyeda88 1 · 0⤊ 1⤋